質(zhì)譜技術(shù)在微生物鑒定和生物醫(yī)學(xué)中的應(yīng)用.ppt

質(zhì)譜技術(shù)在微生物鑒定和生物醫(yī)學(xué)中的應(yīng)用,306醫(yī)院檢驗(yàn)科易勇2016.03,島津（梅里埃）MALDITOF產(chǎn)品系列,LinearonlyMALDI-TOF,LinearandreflectronMALDI-TOFPSDcapability,LinearandreflectronMALDI-TOF/TOFPSDHPLC-microTOFQ）,當(dāng)設(shè)置誤差范圍<5ppm時(shí)：,,,,171個(gè)可能的分子式,GenerateMolecularFormula,,,16個(gè)可能的分子式,當(dāng)設(shè)置誤差范圍<3ppm時(shí)：,GenerateMolecularFormula,,C55H93O25,,用SigmaTM參數(shù)比較同位素峰形匹配，選擇Sigma值最小的分子式，即該化合物的分子式為：,GenerateMolecularFormula,SigmaTM參數(shù)用于比較同位素峰形匹配,,,,,,,,1153.60114,1154.60456,1155.60742,1156.61021,C55H93O25,1153.60,,,,,,,,1153.59527,1154.59868,1155.60170,1156.60459,C62H89O20,1153.59,,0,,,,,,500,,,,,,1000,,,,,,1500,,,,,,2000,,,,,,2500,,,,,,Intens.,,0,,,,,,500,,,,,,1000,,,,,,1500,,,,,,2000,,,,,,2500,,,,,,,,,,1153,,,,,,1154,,,,,,1155,,,,,,1156,,,,,,1157,,,,,,1158,,,,,,1159,,,,,m/z,C55H93O25,C62H89O20,SigmaTM=0.0171,SigmaTM=0.0540,,,,,未知多肽測(cè)序測(cè)定GluFib:MS/MSofm/z=785.8(+2),——?jiǎng)討B(tài)范圍的實(shí)際意義,高質(zhì)量準(zhǔn)確度測(cè)定與液質(zhì)聯(lián)用,常規(guī)高分辨質(zhì)譜儀在液質(zhì)聯(lián)用時(shí)面臨的挑戰(zhàn),,LC/MS高質(zhì)量準(zhǔn)確度測(cè)定在法醫(yī)藥物監(jiān)測(cè)中的應(yīng)用Dept.ofForensicMedicineUniversityofHelsinki,Finland,RapidCommun.MassSpectrum2006;20:1161-1167,尿樣–法醫(yī)毒理分析,總離子流色譜圖TIC,準(zhǔn)確質(zhì)量動(dòng)態(tài)范圍forensicscreening:positivehits,error:2.98ppmsigma:0.0165,error:0.19ppmsigma:0.0084,Signalintensity:8x103,Signalintensity:2x106,布魯克集團(tuán)公司,AffiliatedCompanies,,,,,豐收：土地、種子、氣候......質(zhì)譜儀細(xì)菌鑒定：細(xì)菌純培養(yǎng)、完善的數(shù)據(jù)庫........（耐藥性檢測(cè)、混合菌檢測(cè)）科研：科研設(shè)計(jì)、大量典型的樣品.......種類型號(hào)眾多的質(zhì)譜儀，每種有其特定的用途。,