電針對坐骨神經(jīng)損傷大鼠腦源性神經(jīng)營養(yǎng)因子的影響3800字

電針對坐骨神經(jīng)損傷大鼠腦源性神經(jīng)營養(yǎng)因子的影響3800字 摘要：目的 觀察電針對坐骨神經(jīng)損傷（SNI）大鼠腦源性神經(jīng)營養(yǎng)因子（BDNF）的影響，探討其治療SNI的生物學(xué)機制。方法 選取成年雄性Wistar大鼠50只，分離并切斷大鼠坐骨神經(jīng)后于體式顯微鏡下將兩側(cè)斷端拉入神經(jīng)再生室內(nèi)造模。大鼠隨機分為正常組、假手術(shù)組、模型組和電針組。電針組進行電針治療，連續(xù)28 d。治療結(jié)束后，HE染色觀察神經(jīng)再生，免疫熒光檢測神經(jīng)組織和脊髓BDNF的表達，ELISA檢測血清BDNF的表達。結(jié)果 電針組軸突再生數(shù)量明顯多于模型組，且大體輪廓較清晰；與模型組比較，電針組可促進大鼠神經(jīng)組織、脊髓和血清BDNF的表達（P<0.01）。結(jié)論 電針可能通過上調(diào)神經(jīng)損傷后BDNF的表達，促進大鼠SNI的再生修復(fù)。 畢業(yè)關(guān)鍵詞：電針；坐骨神經(jīng)損傷；腦源性神經(jīng)營養(yǎng)因子；軸突；大鼠DOI：10.3969/j.issn.1005-5304.2017.06.015中圖分類號：R245 文獻標識碼：A 文章編號：1005-5304（2017）06-0060-04Abstract： Objective To explore the effects of electroacupuncture on brain derived neurotrophic factor （BDNF） of rats with sciatic nerve injury （SNI）； To discuss its biological mechanism for treatment of SNI. Methods Fifty adult male Wistar rats were chosen， and the sciatic nerves of rats were cut off and pulled on both sides of the cut ends into nerve regeneration chamber. The rats were randomly divided into normal group， sham-operation group， model group， and electroacupuncture group. In the electroacupuncture group， the rats were treated by electroacupuncture for 28 days. After the treatment， the nerve regeneration was observed through HE staining. Immunofluorescence was used to analyze the expression changes of BDNF in the nerve tissue and spinal cord. ELISA was used to observe the changes of expression of serum BDNF. Results The amount of axon regeneration in the electroacupuncture group was obviously more than that in the model group， and the outline of the tissue more clear. Electroacupuncture could promote the expression of BDNF in the nerve， spinal cord and serum of SNI of rats compared with model group （P<0.01）. Conclusion Electroacupuncture can promote the repairment and regeneration of SNI in rats by upregulating the expression of BDNF.Key words： electroacupuncture； sciatic nerve； brain derived neurotrophic factor； axon； rats周?神經(jīng)損傷（PNI）屬中醫(yī)學(xué)“傷筋”“痿證”范疇。諸病源候論?金瘡傷筋斷骨候云：“夫金瘡始傷之時，半傷其筋，榮衛(wèi)不通，其瘡雖愈合后，仍令痹不仁也?！蹦壳爸委煼椒ㄓ猩窠?jīng)吻合技術(shù)、細胞移植及相關(guān)藥物應(yīng)用等，但神經(jīng)功能的恢復(fù)仍不理想1-2。實驗和臨床研究表明，電針在損傷神經(jīng)的功能恢復(fù)方面療效肯定和優(yōu)勢明顯，能明顯提高PNI患肢的肌力，促進神經(jīng)功能的恢復(fù)，減輕后遺癥3-4。基金項目：國家自然科學(xué)基金（81373754）通訊